Digital Microfluidics Chips for the Execution and Real-Time Monitoring of Multiple Ribozymatic Cleavage Reactions

In this paper, we describe the design and performance of two digital microfluidics (DMF) chips capable executing multiple ribozymatic reactions, with proper controls, in response to short single-stranded DNA inducers. Since fluorescence output a reaction is measurable directly from chip, without need for gel electrophoresis, complete experiment involving up eight reactions (per chip) can be carried out reliably, relatively quickly, efficiently. The ribozymes also used as biosensors concentration oligonucleotide inputs, high sensitivity, low limits quantification detection, excellent signal-to-noise ratio. presented are readily usable devices that automate, speed up, reduce costs experiments.